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The 2 protein bands from 40 kDa to thirty kDa ended up absent immediately after 24 h incubation at forty five °C. For the rest of the bands, different concentrations of all three proteases retained protein bands comparable to their primary molar masses (Figure 6a,b). The existence of bands at very low molecular fat in fish roe just before hydrolysis was unanticipated. While in the report by Ghelichi et al. [8], small peaks of a relatively very low molecular pounds range (3–fourteen kDa) were observed in defatted carp roe homogenate, perhaps as a consequence of an autolytic procedure developing in fish roe and/or even the defatting procedure leading to the development of very low-molecular-weight peptides.
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